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interest goi locus  (New England Biolabs)


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    Structured Review

    New England Biolabs interest goi locus
    Interest Goi Locus, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/interest+goi+locus/Q5+Site-Directed+Mutagenesis+Kit/bio_rxiv__64898__2026__04__08__717193-393-18-27
    Average 99 stars, based on 9775 article reviews
    interest goi locus - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Mitochondrion-IMC contact sites are critical for cofactor biosynthesis and egress signaling in Toxoplasma gondii
    Article Snippet: .. For this purpose, we replaced the guide RNA in pSAG1-Cas9-GFP-pU6-sgUPRT [ ] for one targeting the gene of interest (GOI) locus using the Q5 site-directed mutagenesis kit (NEB). ..

    Article Title: IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion–pellicle contact sites in Toxoplasma gondii
    Article Snippet: .. For this purpose, we replaced the guide RNA in pSAG1-Cas9-GFP-pU6-sgKu80 [modified by ( ) from the original pSAG1-Cas9-GFP-UPRT ( )] for one targeting the gene of interest (GOI) locus using the Q5 site-directed mutagenesis kit (NEB). .. Briefly, freshly egressed parasites were harvested and washed once in PBS and resuspended in transfection buffer (Buffer P3, Lonza), and transferred to transfection cuvettes.

    Modification:

    Article Title: IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion–pellicle contact sites in Toxoplasma gondii
    Article Snippet: .. For this purpose, we replaced the guide RNA in pSAG1-Cas9-GFP-pU6-sgKu80 [modified by ( ) from the original pSAG1-Cas9-GFP-UPRT ( )] for one targeting the gene of interest (GOI) locus using the Q5 site-directed mutagenesis kit (NEB). .. Briefly, freshly egressed parasites were harvested and washed once in PBS and resuspended in transfection buffer (Buffer P3, Lonza), and transferred to transfection cuvettes.



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    99
    New England Biolabs interest goi locus
    Interest Goi Locus, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/interest+goi+locus/Q5+Site-Directed+Mutagenesis+Kit/bio_rxiv__64898__2026__04__08__717193-393-18-27
    Average 99 stars, based on 1 article reviews
    interest goi locus - by Bioz Stars, 2026-09
    99/100 stars
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